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Antibodies
Anti-G-protein coupled receptor 30 antibody
Anti-G-protein coupled receptor 30 antibody from Abcam
Be the first to write a review!
Citations:
(72)
Supplier Page
Supplier Page from
Abcam for
Anti-G-protein coupled receptor 30 antibody
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-G-protein coupled receptor 30 antibody
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab39742
Antibody Type
Primary
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Quantity
100 UG
Conjugate/Tag
Unconjugated
Concentration
0.4 mg/ml
Research Area
Immuno-oncology
Gene Name
GPER1
Reactivity
Human
Target
G-protein coupled receptor 30
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (72)
(1)
PJA1 mediates the effects of astrocytic GPR30 on learning and memory in female mice
Journal of Clinical Investigation
September 15, 2023
Xinshang Wang, Yongli Jiang, Ban Feng, Xue Ma, Kun Zhang, Fan Yang, Zhenguo Liu, Le Yang, Jiao Yue, Liang Lu,
et al.
Cell Signaling Technology
), p-
CREB
(
1:1,000
, no.
9198
,
Cell Signaling Technology
), GPR30 (
1:1,000
, no.
ab39742
,
Abcam
),
AACT
(
1:1,000
, no.
ab205198
,
Abcam
), and
GFAP
(
1:1,000
, no.
3670
,
Cell Signaling Technology
)
(2)
2-Methoxyestradiol as an Antiproliferative Agent for Long-Term Estrogen-Deprived Breast Cancer Cells
Current Issues in Molecular Biology
September 9, 2023
Masayo Hirao-Suzuki, Koki Kanameda, Masufumi Takiguchi, Narumi Sugihara, Shuso Takeda
to that of β-actin. 2.4. Ponceau
Staining
and Western Blot
Analysis Antibodies specific to GPER1 (
ab39742
;
Abcam
, Cambridge, MA, USA), β-actin (
sc-47778
;
Santa Cruz Biotechnology
, Dallas, TX, USA), α-tubulin
(3)
Transcription factor Krüppel‐like factor 4 upregulated G protein‐coupled receptor 30 alleviates intestinal inflammation and apoptosis, and protects intestinal integrity from intestinal ischemia–reperfusion injury
Immunity, Inflammation and Disease
July 27, 2023
Jie Yin, Xiaoli Xie, Jinfeng Yao, Xiaoxu Jin, Huiqing Jiang, Chenguang Ji
After being blocked with skimmed milk, the
membranes
were probed with primary antibodies against GPR30 (
ab39742
,
Abcam
), p‐
p65
(#
3031
,
Cell Signaling Technology
),
p65
(#
8242
,
Cell Signaling Technology
),
Cox2
(
ab179800
,
(4)
Molecular identification of bulbospinal ON neurons by GPER, which drives pain and morphine tolerance
Journal of Clinical Investigation
January 3, 2023
Yingfu Jiao, Po Gao, Li Dong, Xiaowei Ding, Youqiang Meng, Jiahong Qian, Ting Gao, Ruoxi Wang, Tao Jiang, Yunchun Zhang,
et al.
different primary antibodies for 20 hours at 4°C. The primary antibodies rabbit anti-GPER (
1:1,000
,
Abcam
,
ab39742
); rabbit anti-MOR (
1:2,000
,
Immunostar
,
24216
); and
mouse
anti–β-actin (
1:2000
,
MilliporeSigma
,
MAB1501
)
(5)
Overexpression of estrogen receptor GPER1 and G1 treatment reduces SARS-CoV-2 infection in BEAS-2B bronchial cells
Molecular and Cellular Endocrinology
December 1, 2022
Angelica Jardim Costa et al.
(MC-20,
sc-542
,
Santa Cruz Biotechnology
,
1:1000
), ERβ (MC10, Thermo Fisher Scientific,
1:2000
),
GPER1
(
ab39742
,
Abcam
,
1:2000
), ACE2 (
ab15348
,
Abcam
1:2000
) and
TMPRSS2
(
sc515727
,
Santa Cruz Biotechnology
,
1:1000
)
(6)
Icaritin inhibits neuroinflammation in a rat cerebral ischemia model by regulating microglial polarization through the GPER-ERK-NF-κB signaling pathway
Molecular medicine (Cambridge, Mass.)
November 26, 2022
Yu Z, Su G, Zhang L, Liu G, Zhou Y, Fang S, Zhang Q, Wang T, Huang C, Huang Z,
et al
.
Primary antibodies against Iba1 (
ab5076
),
CD40
(
ab13545
),
CD68
(
ab125212
),
CD206
(
ab125028
), and GPER (
ab39742
) were purchased from
Abcam
(Cambridge, UK). Primary antibodies against
P65
-NF-κB (
8242s
), p-
P65
-NF-κB
(7)
GPER limits adverse changes to Ca2+ signalling and arrhythmogenic activity in ovariectomised guinea pig cardiomyocytes
Frontiers in Physiology
November 10, 2022
Alice J. Francis, Jahn M. Firth, Jose L. Sanchez-Alonso, Julia Gorelik, Kenneth T. MacLeod
anti-ERα (
sc-543
,
Santa Cruz Biotechnology
), anti-ERβ (
sc-390243
,
Santa Cruz Biotechnology
), anti-GPER (
ab39742
,
Abcam
) primary antibody diluted in BSA (all
1:150
). The secondary antibody diluted in BSA (
1:400
) (
goat
(8)
Targeted activation of GPER enhances the efficacy of venetoclax by boosting leukemic pyroptosis and CD8+ T cell immune function in acute myeloid leukemia
Cell Death & Disease
October 31, 2022
Jun Ren, Yonghong Tao, Meixi Peng, Qiaoling Xiao, Yipei Jing, Junpeng Huang, Jing Yang, Can Lin, Minghui Sun, Li Lei,
et al.
and then blocked for 25 min at room temperature with 5%
goat
serum. Primary antibodies against
GPER
(
ab39742
,
1:200
,
Abcam
), Phospho-p38 MAPK (#
4511
,
1:1600
,
Cell Signaling Technology
, USA), and Phospho-SAPK/
JNK
(9)
Cytoplasmic G Protein-Coupled Estrogen Receptor 1 as a Prognostic Indicator of Breast Cancer: A Meta-Analysis
Technology in Cancer Research & Treatment
October 18, 2022
Duo Zhang, Jinpeng Wang, Hong Chen, Shunchao Yan
or nucleus,Survival curve,No,0.92 (0.01-106.92) Samartzis. et al,2014,782,Switzerland,Unavailable,
Ab39742
(
Abcam
),
Cytoplasm
or nucleus,Given by author,
Cytoplasm
0.60(0.40-0.9),No Samartzis. et al,2014,782,Switzerland,Unavailable,
Ab39742
(10)
Expression and estrogen regulation of G protein‑coupled estrogen receptor in human glioblastoma cells
Oncology Letters
September 21, 2022
Karla Mariana Peña‑Gutiérrez, Karina Hernández‑Ortega, Claudia Bello‑Alvarez, Ignacio Camacho‑Arroyo
Biotechnology) at 37°C for 2 h.
Membranes
were incubated with the primary antibody against GPER (
1:400
; cat. no.
ab39742
;
Abcam
) at 4°C for 48 h. As a loading control, the α-tubulin protein content (
1:1,000
, at 4°C for 24;
(11)
High GPER expression in triple-negative breast cancer is linked to pro-metastatic pathways and predicts poor patient outcomes
npj Breast Cancer
August 30, 2022
Ting Xu, Ding Ma, Sheng Chen, Rui Tang, Jianling Yang, Chunhui Meng, Yang Feng, Li Liu, Jiangfen Wang, Haojun Luo,
et al.
sections were blocked using
goat
serum and then incubated with the primary antibody targeting GPER (
1:250
,
ab39742
,
Abcam
, USA) at a
1:200
dilution at 4 °C for 16 h. The section treated with PBS worked as a negative
(12)
Proliferation and apoptosis regulation by G protein‑coupled estrogen receptor in glioblastoma C6 cells
Oncology Letters
May 18, 2022
Coral Estefania Gutiérrez‑Almeida, Anne Santerre, Lilia Carolina León‑Moreno, Irene Guadalupe Aguilar‑García, Rolando Castañeda‑Arellano, Sergio Horacio Dueñas‑Jiménez, Judith Marcela Dueñas‑jiménez
incubated overnight at 4°C either with the primary rabbit polyclonal anti-GPER antibody (
1:200
; cat. no.
ab39742
), or the
mouse
monoclonal anti-Ki67 antibody (
1:200
; cat. no.
ab8191
) (both from
Abcam
), or the
mouse
(13)
Role of GPER1 in the Mechanism of EGFR-TKIs Resistance in Lung Adenocarcinoma
Frontiers in Oncology
May 18, 2022
Zhenhua Li, Yaqiang Pan, Qinghua Liu, Jian Wang, Chang Liu, Laihao Qu, Dingbiao Li
primary antibody reaction employed a polyclonal rabbit antibody against GPER1 (
1:200
;
Abcam
, code,
ab39742
), confirmed to be specific for GPER1 (10), for 90 min at room temperature. Thereafter, visualization
(14)
Assessment of G Protein-Coupled Oestrogen Receptor Expression in Normal and Neoplastic Human Tissues Using a Novel Rabbit Monoclonal Antibody
International journal of molecular sciences
May 6, 2022
Bubb M, Beyer AL, Dasgupta P, Kaemmerer D, Sänger J, Evert K, Wirtz RM, Schulz S, Lupp A.
normal and neoplastic tissue samples were also stained with the polyclonal rabbit anti-GPER antibody
ab39742
(
Abcam
, Cambridge, UK), which is directed against the C-terminal tail of
GPER
as well, or with the polyclonal
(15)
Bisphenol A Analogues Suppress Spheroid Attachment on Human Endometrial Epithelial Cells through Modulation of Steroid Hormone Receptors Signaling Pathway
Cells
October 26, 2021
Hongjie Fan, Sudini R. Fernando, Luhan Jiang, Ziyi Wang, Suranga P. Kodithuwakku, Chris K. C. Wong, Ernest H. Y. Ng, William S. B. Yeung, Kai-Fai Lee
antibodies specific for ERα (HC-20, Santa Cruz), ERβ (EPR3778, Abcam), PR (M3569, DAKO), and GPR30 (
ab39742
,
Abcam
) from different sources, followed by anti-rabbit or anti-
mouse
secondary antibody conjugated
(16)
Sex Differences in the Vasodilation Mediated by G Protein-Coupled Estrogen Receptor (GPER) in Hypertensive Rats
Frontiers in Physiology
July 29, 2021
Nathalie Tristão Banhos Delgado, Wender do Nascimento Rouver, Leandro Ceotto Freitas-Lima, Ildernandes Vieira-Alves, Virgínia Soares Lemos, Roger Lyrio dos Dos Santos
in a humid chamber at 4°C overnight with the primary antibodies: rabbit anti-GPER (
1: 400
,
Abcam
, #
ab39742
) and
sheep
anti-VWF (
1:300
,
Abcam
, #
ab11713
). Next,
incubation
was performed for 1 h at room temperature
(17)
G Protein-Coupled Estrogen Receptor 1 (GPER1) Mediates Aldosterone-Induced Endothelial Inflammation in a Mineralocorticoid Receptor-Independent Manner
International Journal of Endocrinology
June 18, 2021
Ziwei Tang, Qifu Li, Qingfeng Cheng, Mei Mei, Ying Song, Zhipeng Du, Wenwen He, Jinbo Hu, Shumin Yang, Zhihong Wang
anti-NOD-, LRR-, and pyrin domaincontaining protein 3 (NLRP-3) (
ab210491
); anti-IL-1β (
ab2105
); anti-GPER1 (
ab39742
); and anti-MR (
ab2774
) antibodies were purchased from
Abcam
, UK. The second antibodies were purchased
(18)
G Protein-Coupled Estrogen Receptor Agonist G-1 Inhibits Mantle Cell Lymphoma Growth in Preclinical Models
Frontiers in Oncology
June 15, 2021
Lixia Zhou et al.
and
negative control siRNA
were purchased from
GenePharma
(Shanghai, China). Antibodies against GPER (
ab39742
), CD20 (
ab9475
), NOX1 (
ab55831
), GAPDH (
ab181602
), anti-mouse IgG H&L (Alexa Fluor 594) (
ab150116
),
(19)
Activation of GPR30 with G1 inhibits oscillatory shear stress-induced adhesion of THP-1 monocytes to HAECs by increasing KLF2
Aging (Albany NY)
April 30, 2021
Chi Chen, Jingyan Chen, Xuefei Tao, Minghuan Fu, Biao Cheng, Xiaohan Chen
X-ray
film (#4741019291, Fujifilm). The following antibodies were used in this study: GPR30 (
1:1000
, #
ab39742
,
Abcam
, USA), KLF2 (
1:1000
, #
ab139699
,
Abcam
, USA), p-
ERK5
(
1:1000
, #
3371
,
Cell Signaling Technology
,
(20)
The neuroendocrine effects of dehydroepiandrosterone and 17β-estradiol in the in vitro preterm hyperoxia infant model
Journal of Integrative Neuroscience
March 30, 2021
Stephanie Hübner, Donna
Anti-β-Actin Peroxidase AffiniPure F(ab’)2 Fragment
Goat
Santa Cruz
, #
sc-543
Affinity BioReagent, #PA1-311 Abcam, #
ab39742
Abcam
, #
ab9474
Sigma-Aldrich
, #A6022
Jackson ImmunoResearch
, #
111-036-003
Anti-Rabbit IgG (H + L) Peroxidase
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Anti-G-protein coupled receptor 30 antibody from Abcam
Supplier Page
View Supplier Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
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